Enzymatic Synthesis of Hydrophobic Penicillins.
نویسندگان
چکیده
منابع مشابه
In vitro enzymatic synthesis of new penicillins containing keto acids as side chains.
Seven different penicillins containing alpha-ketobutyric, beta-ketobutyric, gamma-ketovaleric, alpha-ketohexanoic, delta-ketohexanoic, epsilon-ketoheptanoic, and alpha-ketooctanoic acids as side chains have been synthesized in vitro by incubating the enzymes phenylacetyl coenzyme A (CoA) ligase from Pseudomonas putida and acyl-CoA:6-aminopenicillanic acid acyltransferase from Penicillium chryso...
متن کامل"In vitro" synthesis of different naturally-occurring, semisynthetic and synthetic penicillins using a new and effective enzymatic coupled system.
Forty-seven different penicillins, including some of great clinical importance, have been synthesized "in vitro" by coupling the newly described enzyme phenylacetyl-CoA ligase (PCL) from Pseudomonas putida and acyl-CoA: 6-aminopenicillanic acid (6-APA) acyltransferase (AT) from Penicillium chrysogenum. Incubations were carried out at 30 degrees C in 50 mM HCl-Tris buffer pH 8.0. The reaction mi...
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Paper III (1) in this series describes several tests which were found to be useful in determining whether a given compound is utilized by the mold in penicillin formation. To provide proof of the incorporation of various acyl groups into the penicillin molecule and to be able to determine the chemical characteristics and biological properties of the various penicillins, isolation and crystalliz...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1995
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.48.1195